C18 clean up of peptides using Millipore ZipTips Equipment Millipore C18 ZipTips, cat# ZTC 18S 096, peptide capacity 5 ug or Millipore C18 ZipTips, cat# ZTC 18M 096, peptide capacity 2 ug Put ZipTip on a 10 ul pipettor, wet resin with 10 uL ACN. Discard ACN. 3. Wash ZipTip 2 times with 10 uL 0. 1 TFA, discard washes.
1, set pipette to 10 ul and attach a Milipore C18 ZipTip. 2, equilibrate the ZipTip: pipette up 100 ACN and discard ACN to waste, repeat 3 times, followed by pipetting up Perform ZipTipC18 column (Millipore) cleanup before spotting onto MALDI plate.
References. Saveliev, S. et al Promega Notes 99. 2008; 37. Conventional In Gel Digestion of Proteins [Overnight general protocol for all sample quantities Buffers and Solutions. Gel fragment preparation. Excise protein bands. Cut each into 1 mm pieces. ZipTip sample cleanup Use C18 ZipTip for cleaning up peptide samples Your sample must be in an aqueous buffer to bind to the ZipTip.
The ZipTip is a 10 L (P10) pipette tip with a bed of chromatography media fixed at its end such that there is no dead volume. It is intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality.
The Shop online for a wide selection of EMD Millipore ZipTip Pipet Tips Sample preparation and precise liquid handling in one device Introduction The ZipTip pipette tip is a 10 L pipette tip with a bed of chromatography media fixed at its end.
It is intended for concentrating and purifying peptide, protein or oligonucleotide samples. C18 will not remove detergents, especially the polymeric detergents like NP40, Triton, Tween, Chaps. Filters can help remove some detergent but they cause sample loss. Are ZipTip pipette tips compatible with robotic automated sample preparation systems? ZipTipC18 and ZipTipC4? The ZipTip Operators Manual recommends to aspirate and dispense samples 3 to 7 cycles for simple mixtures and up to 10 Ziptip c18 manual muscle for complex mixtures to achieve maximum binding.
C18 ZipTip Peptide Cleanup Protocol for LCMSMS (NOTE: This will NOT remove detergents) For most applications, use micro ZipTips ((C18) Solution Prep Lecture 4. Sample Preparation for MALDI and LCMS 1. Introduction 2. Sample Preparation for MALDI a. Methods b. Matrix selection c. Sample cleanup for MALDI d.
Matrix cleanup C18 ZipTip pipettes are the tips of choice for peptides and low MW proteins. C8 Zip Tip pipettes are most suitable for low to intermediate MW ZipTip binding, washing and elution are performed utilizing the standard protocols listed in the ZipTip ReversedPhase Operating Manual.
Prior to the final dispense (elution of the peptide or protein) the cut down gel loading tip is pressed on to the end of the ZipTip using a slight twisting motion. ZipTip with 0. 6 L C18 resin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. SDS Technical Information Thermo Scientific Pierce C18 Tips are readytouse pipettetip columns of C18 resin that enable fast and efficient capture, concentration, desalting and elution of peptides for