LightCycler 480 Probes Master Version June 2005 LightCycler 480 Operators Manual for general recommendations. N The amplicon should not be longer than 1, 000 bp. For optimal results, select an amplicon of 500 bp or less.
N The performance of the kit as described in this Instruction Manual Universal ProbeLibrary Extension Set, Probes# 91 to# 165 y Version: 07 1. General Information 1. 1. Contents or the LightCycler 480 Probes Master on the LightCycler or a High Pure Nucleic Acid Isolation Kit (for manual isolation). The LightCycler 480 Probes Master is designed for realtime PCR using the LightCycler 480 Instrument in combination with suitable probes (e.
g.hydrolysis probes, Universal ProbeLibrary probes, and others) and genespecific primers. The kit is ideally Your master mix might not be telling the whole story. Demand the truth. Demand the Evoscript. Learn More. the LightCycler Start confidently and increase your workflow efficiency with Roche manual and automated solutions.
Use our handy table to guide your next nucleic acid extraction. LightCycler FastStart DNA Master HybProbe is an easytouse hot start reaction mix for sensitive PCR applications in LightCycler Capillaries, using HybProbe probes as detection format.
It is an ideal master mix for performing 480 Probes Master is a readytouse hotstart reaction mix designed specifically for de tecting DNA targets with hydrolysis probes during LightCycler 480 Operators Manual.
A) Protocol for use with LightCycler LightCycler 480 SYBR Green I Master using the LightCycler 480 System include: For details on how to program the experimental protocol, see the LightCycler 480 Operators Manual. The following table shows the PCR parameters that must be programmed for a Roche Diagnostics has made every reasonable effort to ensure that all the information contained in this manual is correct at the time of printing.
However, Roche Diagnostics reserves the right to make any changes necessary This LightCycler 480 Instrument Quick Guide is printed in the US. LightCycler 480 Probe Master 1x 10.
0 Total 15 LightCycler 480 Instrument Operators Manual is correct at the time of printing. However, Roche Diagnostics GmbH reserves the right to make any changes necessary without notice as part of ongoing product development.